diff --git a/src/slic3r/GUI/CalibrationWizardPresetPage.cpp b/src/slic3r/GUI/CalibrationWizardPresetPage.cpp
index b4b81b35e..140e467ff 100644
--- a/src/slic3r/GUI/CalibrationWizardPresetPage.cpp
+++ b/src/slic3r/GUI/CalibrationWizardPresetPage.cpp
@@ -1768,6 +1768,18 @@ void CalibrationPresetPage::update_show_status()
         return;
     }
 
+    if (obj_->is_multi_extruders()) {
+        float diameter = obj_->m_extder_data.extders[0].current_nozzle_diameter;
+        bool  is_same_diameter = std::all_of(obj_->m_extder_data.extders.begin(), obj_->m_extder_data.extders.end(),
+           [diameter](const Extder& extruder) {
+                return std::fabs(extruder.current_nozzle_diameter - diameter) < EPSILON;
+           });
+        if (!is_same_diameter) {
+            show_status(CaliPresetPageStatus::CaliPresetStatusDifferentNozzleDiameters);
+            return;
+        }
+    }
+
     // check sdcard when if lan mode printer
     if (obj_->is_lan_mode_printer()) {
         if (obj_->get_sdcard_state() == MachineObject::SdcardState::NO_SDCARD) {
@@ -1897,6 +1909,11 @@ void CalibrationPresetPage::show_status(CaliPresetPageStatus status)
         update_print_status_msg(msg_text, true);
         Enable_Send_Button(false);
     }
+    else if (status == CaliPresetPageStatus::CaliPresetStatusDifferentNozzleDiameters) {
+        wxString msg_text = _L("Calibration only supports cases where the left and right nozzle diameters are identical.");
+        update_print_status_msg(msg_text, true);
+        Enable_Send_Button(false);
+    }
 
     Layout();
 }
diff --git a/src/slic3r/GUI/CalibrationWizardPresetPage.hpp b/src/slic3r/GUI/CalibrationWizardPresetPage.hpp
index 34a377688..d91f9a68b 100644
--- a/src/slic3r/GUI/CalibrationWizardPresetPage.hpp
+++ b/src/slic3r/GUI/CalibrationWizardPresetPage.hpp
@@ -154,6 +154,7 @@ enum CaliPresetPageStatus
     CaliPresetStatusUnsupportedPrinter,
     CaliPresetStatusInConnecting,
     CaliPresetStatusFilamentIncompatible,
+    CaliPresetStatusDifferentNozzleDiameters
 };
 
 class CalibrationPresetPage : public CalibrationWizardPage