diff --git a/src/slic3r/GUI/AMSMaterialsSetting.cpp b/src/slic3r/GUI/AMSMaterialsSetting.cpp
index c938e1052..ed62f0c4e 100644
--- a/src/slic3r/GUI/AMSMaterialsSetting.cpp
+++ b/src/slic3r/GUI/AMSMaterialsSetting.cpp
@@ -570,20 +570,19 @@ void AMSMaterialsSetting::on_select_ok(wxCommandEvent &event)
 
 
                 //check is it in the filament blacklist
-                if (!is_virtual_tray() && wxGetApp().app_config->get("skip_ams_blacklist_check") != "true") {
+                if (wxGetApp().app_config->get("skip_ams_blacklist_check") != "true") {
                     bool in_blacklist = false;
                     std::string action;
                     std::string info;
                     std::string filamnt_type;
                     it->get_filament_type(filamnt_type);
 
-                    auto vendor = dynamic_cast<ConfigOptionStrings*> (it->config.option("filament_vendor"));
+                    auto vendor = dynamic_cast<ConfigOptionStrings *>(it->config.option("filament_vendor"));
                     if (vendor && (vendor->values.size() > 0)) {
                         std::string vendor_name = vendor->values[0];
-                        DeviceManager::check_filaments_in_blacklist(vendor_name, filamnt_type, in_blacklist, action, info);
+                        DeviceManager::check_filaments_in_blacklist(vendor_name, filamnt_type, ams_id, in_blacklist, action, info);
                     }
 
-
                     if (in_blacklist) {
                         if (action == "prohibition") {
                             MessageDialog msg_wingow(nullptr, wxString::FromUTF8(info), _L("Error"), wxICON_WARNING | wxOK);
diff --git a/src/slic3r/GUI/CalibrationWizardPage.cpp b/src/slic3r/GUI/CalibrationWizardPage.cpp
index d07a04ad8..4e26bd316 100644
--- a/src/slic3r/GUI/CalibrationWizardPage.cpp
+++ b/src/slic3r/GUI/CalibrationWizardPage.cpp
@@ -278,13 +278,14 @@ FilamentComboBox::FilamentComboBox(wxWindow* parent, const wxPoint& pos, const w
 void FilamentComboBox::ShowPanel()
 {
     this->Show();
-    m_radioBox->Show();
+    set_select_mode(m_mode);
 }
 
 void FilamentComboBox::HidePanel()
 {
     this->Hide();
     m_radioBox->Hide();
+    m_checkBox->Hide();
 }
 
 void FilamentComboBox::set_select_mode(CalibrationFilamentMode mode)
diff --git a/src/slic3r/GUI/CalibrationWizardPresetPage.cpp b/src/slic3r/GUI/CalibrationWizardPresetPage.cpp
index 5c8a00011..70f463781 100644
--- a/src/slic3r/GUI/CalibrationWizardPresetPage.cpp
+++ b/src/slic3r/GUI/CalibrationWizardPresetPage.cpp
@@ -1249,13 +1249,13 @@ void CalibrationPresetPage::check_filament_compatible()
     std::string error_tips;
     int bed_temp = 0;
 
-    std::vector<Preset*> selected_filaments_list;
-    for (auto& item: selected_filaments)
+    std::vector<Preset *> selected_filaments_list;
+    for (auto &item : selected_filaments)
         selected_filaments_list.push_back(item.second);
 
     check_filament_cali_reliability(selected_filaments_list);
 
-    if (!is_filaments_compatiable(selected_filaments_list, bed_temp, incompatiable_filament_name, error_tips)) {
+    if (!is_filaments_compatiable(selected_filaments, bed_temp, incompatiable_filament_name, error_tips)) {
         m_tips_panel->set_params(0, 0, 0.0f);
         if (!error_tips.empty()) {
             wxString tips = from_u8(error_tips);
@@ -1276,7 +1276,7 @@ void CalibrationPresetPage::check_filament_compatible()
     Layout();
 }
 
-bool CalibrationPresetPage::is_filaments_compatiable(const std::vector<Preset*>& prests)
+bool CalibrationPresetPage::is_filaments_compatiable(const std::map<int, Preset*>& prests)
 {
     std::string incompatiable_filament_name;
     std::string error_tips;
@@ -1284,9 +1284,9 @@ bool CalibrationPresetPage::is_filaments_compatiable(const std::vector<Preset*>&
     return is_filaments_compatiable(prests, bed_temp, incompatiable_filament_name, error_tips);
 }
 
-bool CalibrationPresetPage::is_filament_in_blacklist(Preset* preset, std::string& error_tips)
+bool CalibrationPresetPage::is_filament_in_blacklist(int tray_id, Preset* preset, std::string& error_tips)
 {
-    if (m_ams_radiobox->GetValue() && wxGetApp().app_config->get("skip_ams_blacklist_check") != "true") {
+    if (!m_ext_spool_radiobox->GetValue() && wxGetApp().app_config->get("skip_ams_blacklist_check") != "true") {
         bool in_blacklist = false;
         std::string action;
         std::string info;
@@ -1296,7 +1296,7 @@ bool CalibrationPresetPage::is_filament_in_blacklist(Preset* preset, std::string
         auto vendor = dynamic_cast<ConfigOptionStrings*> (preset->config.option("filament_vendor"));
         if (vendor && (vendor->values.size() > 0)) {
             std::string vendor_name = vendor->values[0];
-            DeviceManager::check_filaments_in_blacklist(vendor_name, filamnt_type, in_blacklist, action, info);
+            DeviceManager::check_filaments_in_blacklist(vendor_name, filamnt_type, tray_id, in_blacklist, action, info);
         }
 
         if (in_blacklist) {
@@ -1326,7 +1326,7 @@ bool CalibrationPresetPage::is_filament_in_blacklist(Preset* preset, std::string
     return true;
 }
 
-bool CalibrationPresetPage::is_filaments_compatiable(const std::vector<Preset*> &prests,
+bool CalibrationPresetPage::is_filaments_compatiable(const std::map<int, Preset*> &prests,
     int& bed_temp,
     std::string& incompatiable_filament_name,
     std::string& error_tips)
@@ -1336,22 +1336,23 @@ bool CalibrationPresetPage::is_filaments_compatiable(const std::vector<Preset*>
     bed_temp = 0;
     std::vector<std::string> filament_types;
     for (auto &item : prests) {
-        if (!item)
+        const auto& item_preset = item.second;
+        if (!item_preset)
             continue;
 
         // update bed temperature
         BedType curr_bed_type = BedType(m_comboBox_bed_type->GetSelection() + btDefault + 1);
-        const ConfigOptionInts *opt_bed_temp_ints = item->config.option<ConfigOptionInts>(get_bed_temp_key(curr_bed_type));
+        const ConfigOptionInts *opt_bed_temp_ints = item_preset->config.option<ConfigOptionInts>(get_bed_temp_key(curr_bed_type));
         int bed_temp_int = 0;
         if (opt_bed_temp_ints) {
             bed_temp_int = opt_bed_temp_ints->get_at(0);
         }
 
         if (bed_temp_int <= 0) {
-            if (!item->alias.empty())
-                incompatiable_filament_name = item->alias;
+            if (!item_preset->alias.empty())
+                incompatiable_filament_name = item_preset->alias;
             else
-                incompatiable_filament_name = item->name;
+                incompatiable_filament_name = item_preset->name;
 
             return false;
         } else {
@@ -1360,10 +1361,10 @@ bool CalibrationPresetPage::is_filaments_compatiable(const std::vector<Preset*>
                 bed_temp = bed_temp_int;
         }
         std::string display_filament_type;
-        filament_types.push_back(item->config.get_filament_type(display_filament_type, 0));
+        filament_types.push_back(item_preset->config.get_filament_type(display_filament_type, 0));
 
         // check is it in the filament blacklist
-        if (!is_filament_in_blacklist(item, error_tips))
+        if (!is_filament_in_blacklist(item.first, item_preset, error_tips))
             return false;
     }
 
@@ -2153,14 +2154,16 @@ void CalibrationPresetPage::select_default_compatible_filament()
         return;
 
     if (m_ams_radiobox->GetValue()) {
-        std::vector<Preset*> multi_select_filaments;
+        std::map<int, Preset *> selected_filament;
         for (auto &fcb : m_filament_comboBox_list) {
             if (!fcb->GetRadioBox()->IsEnabled())
                 continue;
-
+            int tray_id = fcb->get_tray_id();
             Preset* preset = const_cast<Preset *>(fcb->GetComboBox()->get_selected_preset());
             if (m_cali_filament_mode == CalibrationFilamentMode::CALI_MODEL_SINGLE) {
-                if (preset && is_filaments_compatiable({preset})) {
+                selected_filament.clear();
+                selected_filament[tray_id] = preset;
+                if (preset && is_filaments_compatiable(selected_filament)) {
                     fcb->GetRadioBox()->SetValue(true);
                     wxCommandEvent event(wxEVT_RADIOBUTTON);
                     event.SetEventObject(this);
@@ -2174,9 +2177,9 @@ void CalibrationPresetPage::select_default_compatible_filament()
                     fcb->GetCheckBox()->SetValue(false);
                     continue;
                 }
-                multi_select_filaments.push_back(preset);
-                if (!is_filaments_compatiable(multi_select_filaments)) {
-                    multi_select_filaments.pop_back();
+                selected_filament.insert(std::make_pair(tray_id, preset));
+                if (!is_filaments_compatiable(selected_filament)) {
+                    selected_filament.erase(tray_id);
                     fcb->GetCheckBox()->SetValue(false);
                 }
                 else
@@ -2190,8 +2193,10 @@ void CalibrationPresetPage::select_default_compatible_filament()
         }
     }
     else if (m_ext_spool_radiobox->GetValue()){
+        std::map<int, Preset *> selected_filament;
         Preset *preset = const_cast<Preset *>(m_virtual_tray_comboBox->GetComboBox()->get_selected_preset());
-        if (preset && is_filaments_compatiable({preset})) {
+        selected_filament[m_virtual_tray_comboBox->get_tray_id()] = preset;
+        if (preset && is_filaments_compatiable(selected_filament)) {
             m_virtual_tray_comboBox->GetRadioBox()->SetValue(true);
         } else
             m_virtual_tray_comboBox->GetRadioBox()->SetValue(false);
diff --git a/src/slic3r/GUI/CalibrationWizardPresetPage.hpp b/src/slic3r/GUI/CalibrationWizardPresetPage.hpp
index 4df430434..a6d4c5128 100644
--- a/src/slic3r/GUI/CalibrationWizardPresetPage.hpp
+++ b/src/slic3r/GUI/CalibrationWizardPresetPage.hpp
@@ -245,9 +245,9 @@ protected:
 
     void check_nozzle_diameter_for_auto_cali();
     void check_filament_compatible();
-    bool is_filaments_compatiable(const std::vector<Preset*>& prests);
-    bool is_filament_in_blacklist(Preset* preset, std::string& error_tips);
-    bool is_filaments_compatiable(const std::vector<Preset*>& prests,
+    bool is_filaments_compatiable(const std::map<int, Preset *>& prests);
+    bool is_filament_in_blacklist(int tray_id, Preset* preset, std::string& error_tips);
+    bool is_filaments_compatiable(const std::map<int, Preset *> &prests,
         int& bed_temp,
         std::string& incompatiable_filament_name,
         std::string& error_tips);
diff --git a/src/slic3r/GUI/DeviceManager.cpp b/src/slic3r/GUI/DeviceManager.cpp
index 0f3a1e53d..43bb4c4f9 100644
--- a/src/slic3r/GUI/DeviceManager.cpp
+++ b/src/slic3r/GUI/DeviceManager.cpp
@@ -257,6 +257,15 @@ PrinterArch get_printer_arch_by_str(std::string arch_str)
     return PrinterArch::ARCH_CORE_XY;
 }
 
+void check_filaments_for_vt_slot(const std::string &tag_vendor, const std::string &tag_type, int ams_id, bool &in_blacklist, std::string &ac, std::string &info)
+{
+    if (tag_type == "TPU" && ams_id != VIRTUAL_TRAY_MAIN_ID) {
+        ac           = "prohibition";
+        info         = wxString(_L("TPU is not supported by deputy extruder.")).ToUTF8().data();
+        in_blacklist = true;
+    }
+}
+
 void AmsTray::update_color_from_str(std::string color)
 {
     if (color.empty()) return;
@@ -6616,8 +6625,20 @@ bool DeviceManager::load_filaments_blacklist_config()
     return true;
 }
 
-void DeviceManager::check_filaments_in_blacklist(std::string tag_vendor, std::string tag_type, bool& in_blacklist, std::string& ac, std::string& info)
+bool DeviceManager::is_virtual_slot(int ams_id)
 {
+    if (ams_id == VIRTUAL_TRAY_MAIN_ID || ams_id == VIRTUAL_TRAY_DEPUTY_ID)
+        return true;
+    return false;
+}
+
+void DeviceManager::check_filaments_in_blacklist(std::string tag_vendor, std::string tag_type, int ams_id, bool& in_blacklist, std::string& ac, std::string& info)
+{
+    if (DeviceManager::is_virtual_slot(ams_id)) {
+        check_filaments_for_vt_slot(tag_vendor, tag_type, ams_id, in_blacklist, ac, info);
+        return;
+    }
+
     std::unordered_map<std::string, wxString> blacklist_prompt =
     {
         {"TPU: not supported", _L("TPU is not supported by AMS.")},
diff --git a/src/slic3r/GUI/DeviceManager.hpp b/src/slic3r/GUI/DeviceManager.hpp
index aea8ea57f..5eafd45ed 100644
--- a/src/slic3r/GUI/DeviceManager.hpp
+++ b/src/slic3r/GUI/DeviceManager.hpp
@@ -1164,8 +1164,11 @@ public:
     static void check_filaments_in_blacklist(std::string tag_vendor, std::string tag_type, bool& in_blacklist, std::string& ac, std::string& info);
     static std::vector<std::string> get_resolution_supported(std::string type_str);
     static std::vector<std::string> get_compatible_machine(std::string type_str);
+    static bool load_filaments_blacklist_config();
+    static void check_filaments_in_blacklist(std::string tag_vendor, std::string tag_type, int ams_id, bool &in_blacklist, std::string &ac, std::string &info);
     static boost::bimaps::bimap<std::string, std::string> get_all_model_id_with_name();
     static std::string load_gcode(std::string type_str, std::string gcode_file);
+    static bool is_virtual_slot(int ams_id);
 };
 
 // change the opacity
diff --git a/src/slic3r/GUI/PartPlate.hpp b/src/slic3r/GUI/PartPlate.hpp
index b37234504..3abed6287 100644
--- a/src/slic3r/GUI/PartPlate.hpp
+++ b/src/slic3r/GUI/PartPlate.hpp
@@ -423,7 +423,7 @@ public:
         bool result = m_slice_result_valid;
         if (result)
             result = m_gcode_result ?
-            (!m_gcode_result->toolpath_outside && !m_gcode_result->filament_printable_reuslt.has_value()) :
+            (!m_gcode_result->toolpath_outside && m_gcode_result->gcode_check_result.error_code == 0 && !m_gcode_result->filament_printable_reuslt.has_value()) :
             false;// && !m_gcode_result->conflict_result.has_value()  gcode conflict can also print
         return result;
     }
diff --git a/src/slic3r/GUI/SelectMachine.cpp b/src/slic3r/GUI/SelectMachine.cpp
index 09b5d52bb..b5a9984a5 100644
--- a/src/slic3r/GUI/SelectMachine.cpp
+++ b/src/slic3r/GUI/SelectMachine.cpp
@@ -2767,8 +2767,7 @@ void SelectMachineDialog::on_ok_btn(wxCommandEvent &event)
         bool in_blacklist = false;
         std::string action;
         std::string info;
-
-        DeviceManager::check_filaments_in_blacklist(filament_brand, filament_type, in_blacklist, action, info);
+        DeviceManager::check_filaments_in_blacklist(filament_brand, filament_type, tid, in_blacklist, action, info);
 
         if (in_blacklist && action == "warning") {
             wxString prohibited_error = wxString::FromUTF8(info);
@@ -2833,8 +2832,7 @@ void SelectMachineDialog::on_ok_btn(wxCommandEvent &event)
         bool in_blacklist = false;
         std::string action;
         std::string info;
-
-        DeviceManager::check_filaments_in_blacklist(filament_brand, filament_type, in_blacklist, action, info);
+        DeviceManager::check_filaments_in_blacklist(filament_brand, filament_type, tid, in_blacklist, action, info);
 
         if (in_blacklist && action == "prohibition") {
             has_prohibited_filament = true;